→ Real-time PCR (qPCR) is a powerful and rapid tool for the specific detection of bacteria in water.
This methodology, widely used in most countries, is already the subject of an ISO standard for the research of Legionella spp and Legionella pneumophila in sanitary and industrial waters. It is based on the amplification and detection of specific genomic sequences. Quantification of the target DNA is performed using a specific probe labelled with the fluorophore FAM. For each amplification reaction, a synthetic DNA serves as an internal control and thus makes it possible to control the absence of inhibition and the successful running of the amplification. This internal control is quantified by a probe coupled to the HEX fluorophore. The DNA AMPLI-FLASH qPCR kit contains ready-to-use reagents for completion of the analysis.
→ The DNA AMPLI-FLASH kit, combined with the DNA PURE-FLASH kit, provides a complete, robust and reliable approach that allows the detection and quantification of Legionella in water.